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interleukin 1 receptor antagonist  (R&D Systems)


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    Structured Review

    R&D Systems interleukin 1 receptor antagonist
    Interleukin 1 Receptor Antagonist, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1ra/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pm42670725-36-34-46
    Average 94 stars, based on 48 article reviews
    interleukin 1 receptor antagonist - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Knockdown:

    Article Title: Newly defined ABCB5 + dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Article Snippet: ABCB5 + -derived MSCs were transiently transfected with 20 nM of either a combination of four siRNAs specific for human IL-1RA or with scrambled control-A siRNA with accompanying transfection medium at the minimum recommended concentration (all products from Santa Cruz Biotechnologies) according to manufacturer’s instructions. .. Successful knock-down was tested at the protein level upon in vitro inflammatory stimulation with IFN-γ/LPS activated mouse bone marrow-derived macrophages and culture supernatant medium ELISA for human IL-1RA (R&D Systems) before use in in vivo experiments and was typically at ~ 80% as shown in . ..

    In Vitro:

    Article Title: Newly defined ABCB5 + dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Article Snippet: ABCB5 + -derived MSCs were transiently transfected with 20 nM of either a combination of four siRNAs specific for human IL-1RA or with scrambled control-A siRNA with accompanying transfection medium at the minimum recommended concentration (all products from Santa Cruz Biotechnologies) according to manufacturer’s instructions. .. Successful knock-down was tested at the protein level upon in vitro inflammatory stimulation with IFN-γ/LPS activated mouse bone marrow-derived macrophages and culture supernatant medium ELISA for human IL-1RA (R&D Systems) before use in in vivo experiments and was typically at ~ 80% as shown in . ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Newly defined ABCB5 + dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Article Snippet: ABCB5 + -derived MSCs were transiently transfected with 20 nM of either a combination of four siRNAs specific for human IL-1RA or with scrambled control-A siRNA with accompanying transfection medium at the minimum recommended concentration (all products from Santa Cruz Biotechnologies) according to manufacturer’s instructions. .. Successful knock-down was tested at the protein level upon in vitro inflammatory stimulation with IFN-γ/LPS activated mouse bone marrow-derived macrophages and culture supernatant medium ELISA for human IL-1RA (R&D Systems) before use in in vivo experiments and was typically at ~ 80% as shown in . ..

    Article Title: Intrathecal Administration of Conditioned Serum from Different Species Resolves Chemotherapy-Induced Neuropathic Pain in Mice via Secretory Exosomes
    Article Snippet: .. ELISA kits for human IL-1Ra (Catlog#DRA00B) and TIMP-1 (Catlog#DY970-05), Human/Mouse/Rat/Porcine/Canine TGF-β (Catlog#DB100B) were purchased from R&D system. .. ELISA kits for RvD1 (Catlog#500380) and RvD2 (Catlog#501120) were purchased from Cayman Chemical.

    Article Title: Intrathecal administration of conditioned serum from different species resolves Chemotherapy-Induced neuropathic pain in mice via secretory exosomes.
    Article Snippet: .. ELISA kits for human IL-1Ra (Catlog#DRA00B) and TIMP-1 (Catlog#DY970-05), Human/Mouse/Rat/Porcine/Canine TGF-β (Catlog#DB100B) were purchased from R&D Systems. .. ELISA kits for RvD1 (Catlog#500380) and RvD2 (Catlog#501120) were purchased from Cayman Chemical.

    Article Title: Intrathecal Administration of Conditioned Serum from Different Species Resolves Chemotherapy-Induced Neuropathic Pain in Mice via Secretory Exosomes
    Article Snippet: .. ELISA ELISA kits for human IL-1Ra (Catlog#DRA00B) and TIMP-1 (Catlog#DY970-05), Human/Mouse/Rat/Porcine/Canine TGF-β (Catlog#DB100B) were purchased from R&D system. .. ELISA kits for RvD1 (Catlog#500380) and RvD2 (Catlog#501120) were purchased from Cayman Chemical.

    In Vivo:

    Article Title: Newly defined ABCB5 + dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Article Snippet: ABCB5 + -derived MSCs were transiently transfected with 20 nM of either a combination of four siRNAs specific for human IL-1RA or with scrambled control-A siRNA with accompanying transfection medium at the minimum recommended concentration (all products from Santa Cruz Biotechnologies) according to manufacturer’s instructions. .. Successful knock-down was tested at the protein level upon in vitro inflammatory stimulation with IFN-γ/LPS activated mouse bone marrow-derived macrophages and culture supernatant medium ELISA for human IL-1RA (R&D Systems) before use in in vivo experiments and was typically at ~ 80% as shown in . ..

    Incubation:

    Article Title: Safety, tolerability and pharmacokinetics and pharmacodynamics of HL2351, a novel hybrid fc‐fused interleukin‐1 receptor antagonist, in healthy subjects: A first‐in‐human study
    Article Snippet: As a detection antibody for Anakinra, a polyclonal antibody specific for human IL‐1ra (R&D Systems Inc., Minneapolis, MN, USA) was added to the plate and incubated for 2 hours at 25°C. .. As a detection antibody for Anakinra, a polyclonal antibody specific for human IL‐1ra (R&D Systems Inc., Minneapolis, MN, USA) was added to the plate and incubated for 2 hours at 25°C. ..

    Article Title: Fusion protein with half-life extending polypeptide
    Article Snippet: Serial dilutions of samples in steps of 1/5 were prepared in a polypropylene plate (Corning 3365) using a liquid handling robot (Biomek 4000, Beckman Coulter). .. Unbound blocking protein was removed by washing with 2×150 μl PBS-Tween and 25 μl of samples and standards were pipetted to the wells and incubated for one hour RT with 600 rpm shake followed by an overnight incubation at +4° C. After washing away any unbound substances with 3×150 μl PBS-Tween, 50 μl of a Biotin conjugated polyclonal detection antibody specific for human IL-1RA (BAF280, R&D Systems) was added to the wells and incubated for 2 hours RT. ..

    Blocking Assay:

    Article Title: Fusion protein with half-life extending polypeptide
    Article Snippet: Serial dilutions of samples in steps of 1/5 were prepared in a polypropylene plate (Corning 3365) using a liquid handling robot (Biomek 4000, Beckman Coulter). .. Unbound blocking protein was removed by washing with 2×150 μl PBS-Tween and 25 μl of samples and standards were pipetted to the wells and incubated for one hour RT with 600 rpm shake followed by an overnight incubation at +4° C. After washing away any unbound substances with 3×150 μl PBS-Tween, 50 μl of a Biotin conjugated polyclonal detection antibody specific for human IL-1RA (BAF280, R&D Systems) was added to the wells and incubated for 2 hours RT. ..



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    Characterization <t>of</t> <t>IL-1α</t> overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, <t>ELISA</t> and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
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    Characterization <t>of</t> <t>IL-1α</t> overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, <t>ELISA</t> and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
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    Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

    Journal: Redox Biology

    Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

    doi: 10.1016/j.redox.2026.104172

    Figure Lengend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

    Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

    Techniques: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control

    IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

    Journal: Redox Biology

    Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

    doi: 10.1016/j.redox.2026.104172

    Figure Lengend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

    Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

    Techniques: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot